Isolation and study of chemical properties of pyocyanin produced from Pseudomonas aeruginosa TISTR 781 (ATCC 9027)(Thai)

Abstract

Blue antibiotic can be produced mainly by Pseudomonas aeruginosa TESTR 781 (ATCC 9.027) when grown in King's A medium (KA). After isolation and purification by both Amberlite XAD-16 resin column and subsequently silica gel column chromatography, bands on the silica gel column that appeared were bright yellow, dark blue and dark green. The purified blue pigment was examined for its homogeneity by TLC and HPLC. According to UV data, the isolated blue band could be pyocyanin whereas the bright yellow could be 1-hydroxyphenazine. A study on the polarity of pyocyanin by varying solvents for TLC indicated that the mixture of dichloromethane-methanol (1:1, w/v) is an optimum solvent. The effects of storage time on structural stability and pH on maximum absorption of pyocyanin have also been studied.