A dtudy on DNA fingerprint of some Thai native frogs using RAPD technique(Thai)

Abstract

A study on DNA fingerprint of some 4 Thai native frogs was to verify species of frogs; 1) Rana tigerina; 2) R. rugulosa; 3) R. leucomystax; 4) R. limnocharis by RAPD technique. The results after PCR-RAPD manipulation indicated 190 DNA band and sizes of DNA bands were between 500-1031 base pairs. The polymorphic band was between 36.36 - 54.55 %. In term of genetic diversity, the similarity index of Thai native frogs was between 0.57-0.80 and genetic distance was between 0.20-0.55