Determination of aflatoxin B1, B2 G, and G2 in chilli paste by high performance liquid chromatography(Thai)

Abstract

In this study aflatoxin B1 , B2 , G1 and G2 in chili paste samples were determined by high performance liquid chromatography (HPLC). The optimum conditions for aflatoxin determination were 0.5 % trifluoroacetic acid : acetonitrile : methanol (65 : 20 : 15) as mobile phase with a suitable flow rate of 1.5 ml min-1 and fluorescence detector with the excitation and emission wavelengths of 324 and 424 nm, respectively. The 76 samples of chili paste samples were collected from markets in Amphur Muang, Khon Kaen Province. The food samples were extracted with chloroform and derivatized with trifluoroacetic acid after clean-up by column chromatography. Under optimum conditions satisfactory separation of aflatoxins G1 , B1 , G2 and B2 was obtained with the retention times of 6.40, 8.24, 11.71 and 16.30 minutes, respectively. The results of aflatoxin B1 , B2 , G1 and G2 level of samples were found to be in the range of 0.0-12.6, 0.0-0.0, 0.0-226.9 and 0.0-38.4 µg kg-1, respectively.