Optimization for Cyclodextrin-Glucanotransferase (CGTase) production by an Alkalophilic Bacterium, Bacillus sp.

Abstract

A new strain of alkalophilic bacterial isolate #10 isolated from Khon Kaen University soil was studied for its optimal condition for CGTase production. The characterization of enzyme production showed an optimal temperature and incubation time of 37 o C and 72 hrs, respectively. The results showed that sticky-rice starch was the best carbon source for enzyme production at a concentration of 1% (w/v). The optimal temperature for the enzyme-catalyzed reaction was 50 o C. The sticky-rice starch was also the best substrate for enzyme activity at a concentration of 4% (w/v). The alkalophilic isolate #10 produced only β-CD as indicated by paper chromatography. From morphology and biochemical tests, this bacterium was identified as Bacillus sp.