Production and characterization of sophorolipids produced by Candida bombicola grown on sugarcane molasses and coconut oil
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Abstract
Sophorolipids (SLs) are a type of biosurfactant belonging to the glycolipid group, which have various applications in many fields. They have been produced to replace chemical surfactants due to their excellent properties including higher biodegradability, lower toxicity and environmental friendliness. However, to compete with more inexpensive chemical surfactants, it is important to use low cost materials for their production. In this study, we produced SLs using Candida bombicola grown on sugarcane molasses and coconut oil. The results showed that the optimal conditions for SL production were 10% (w/v) sugarcane molasses, 10% (v/v) coconut oil, 25oC, pH 6 and 7 days of cultivation. SLs were to resistant E. coli NRRL B-409, P. aeruginosa NRRL B-14781, S. aureus NRRL B-313 and B. subtilis NRRL B-354. They also showed the capability of free radical scavenging in a dose dependent manner with an EC50 value of 0.35 mg/ml with emulsification activity. These results showed that sugarcane molasses and coconut oil could be used for SL production to reduce substrate costs.
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References
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