Relationship between floret size and anther culture response in an ornamental sunflower
Article Sidebar
Main Article Content
Abstract
A correlative approach using light microscope and electron microscope was carried out in this study to assess floret characteristics and microspore developmental stages in an ornamental sunflower cv. Prado Red. The relationship between microspore stage with anther culture response on various culture media was also investigated. The results revealed a decrease in the percentages of mid-to late- uninucleate microspores from 39.33% of the outer unopened disk floret whorl 1 to 15.33% of the inner floret whorl 3. Disk floret length and anther size were positively correlated with the microspore stage. The callus induction was significantly affected by culture medium but not disk floret whorls and the interaction between them, while the percentage of embryogenic calli was significantly affected by culture media and floret whorls but not the interaction between them. Among the four tested media, MS medium supplemented with 2 mg l-1 α-napthalene acetic acid (NAA), 1 mg l-1 N6-benzylaminopurine (BAP) and 100 ml l-1 coconut water produced the highest callus induction (60.44%) and embryogenic callus production (20.45%). Hence larger disk florets with higher percentages of mid-to late- uninucleate microspores responded well and produced embryogenic callus in the optimum culture medium.
Article Details
References
Nascimento AMPD, Reis SN, Nery FC, Curvelo ICS, da Cruz Taquues T, Almeida EFA. Influence of color shading nets on ornamental sunflower development. Ornamental Horticulture. 2016; 22(1): 101-106.
Kaya Y, Jocic S, Miladinovic D. Sunflower. In: Gupta, S. K, editor.Technological innovations in major world oil crops: Breeding, 1st ed. Springer, New York: 2012; 85-130.
Miladanovic E, Cvejic S, Cukanovic J, Zeravica G, Jocic S. Evaluation of sunflower genotypes for ornamental use. Contemporary Agriculture. 2016; 65 (1-2): 39-43.
Cvejic S, Jocic S. Development of ornamental sunflower hybrids. Field Crops Res. 2010; 47: 147-152.
Cvejic S, Jocic S, Milandenovic E. Inheritance of floral colour and type in four new inbred lines of ornamental sunflower (Helianthus annuus L.).J. Hortic. Sci. Biotech. 2016; 91(1): 30-35.
Guha S, Mahesshwari SC. In vitro production of embryo from anther of Datura. Nature. 1964; 204-497.
Todorava M, Dahlhofl M, Friedt W. Microspore culture in sunflower (Helianthus annuus L.). Biotechnol. Biotechnol. Equip. 1993; 7: 80-90.
Thengane SR, Joshi MS, Khuspe SS, Mascarenhas AF. Anther culture in Helianthus annuus L., influence of genotype and culture conditions on embryo induction and plant regeneration. Plant Cell Rep. 1994; 13: 222-226.
Saji KV, Sujath M. Embryogenesis and plant regeneration in anther culture of sunflower (Helianthus annuus L.). Euphytica. 1998; 103: 1-7.
Vijaya Priya VK, Sassikumar D, Sudhager R, Gopalan A. Androgenetic response of sunflower in different culture environment. Helia. 2003; 26: 39-50.
Sujatha M, Pabakaran AJ. Ploidy manipulation and introgression of resistance to Alternaria helianthi from wild hexaploid Helianthus species to cultivated sunflower (H. annuus L.) aided by anther culture. Euphytica. 2006; 152: 201-215.
Dogan E, Akgul N, Aydin Y, Uncuoglu AA, Evci G. Microspore culture response of sunflower (Helianthus annuus L.) cultivars. In: proceeding of the nineteenth International Sunflower Conference. Edirne, Turkey. 2006; P 609
Miladinovic D, Kovacevic B, Jocic B, Imerovski I, Dimitrijevic A, Cvejic S, Balalic I. The effect of sowing date and density on callus induction and shoot regeneration from sunflower anthers. In: proceeding of the nineteenth International Sunflower Conference. Edirne, Turkey. 2016; P 524-529.
Vasilejevic L, Atlagic J, Skoric D. Applicability of new biotechnological methods in sunflower breeding. Ann. Biol. 1990; 6(2): 147-152.
Gurel A, Nichterlein K, Friedt W. Shoot regeneration from anther culture of sunflower (Helianthus annuus L.) and some interspecific hybrids is affected by genotype and culture procedure. Plant Breed. 1991; 106: 68-76.
Elaleen KMA, Saeeed BEAE, Ahmed MM. Effect of plant growth regulators on Helianthus annuus L. callus induction. International Journal of Innovation and Applied Studies. 2015; 13(2): 348-354.
Spurr AR. A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 1969; 26: 31-43
Chahaffey NJ, Cholewa E, Regan S, Sundberg B. Secondary xylem development in Arabidopsis: A model for wood formation. Physiol. Plant. 2002; 114: 594-600.
Venable JH, Coggehall R. A simplified lead citrate stain for use in electron microscopy. J. Cell Biol. 1965; 25: 407-408.
Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 1962; 15: 473-497.
Phaosang T, Malumpong C, Kaweeta R. In vitro differentiation of embryogenesis callus from anther culture of sunflower (Helianthus annuus L.) cv. Kwangsri. In: Proceeding of the fortieth Kasetsart University Annual Conference: Plants, Agricultural Extension and Communication, Agro-Industry. 2003; P 52-63.
Kılıc E, Akgül A, Aydın Y, Uncuoğlu AA. Genotoxic effects of in vitro tissue culture conditions in sunflower (Helianthus annuus L.). In: proceeding of the Nineteenth International Sunflower Conference, Edirne, Turkey. 2016; P 610.
Summer WL, Jaramillo J, Bailey T. Microspore developmental stage and anther length influence the induction of tomato anther callus. HortScience. 1992; 27(7): 838-840.
Croser JS, Lulsdorf MM, Grewal RW, Usher KM, Siddique KHM. Isolated microspore culture of chickpea (Cicer arietinum): induction of androgenesis and cytological analysis of early haploid divisions. In. Vitro. Cell. Dev. Biol. Plan. 2001; 47: 357-368.
Barroso PA, Rego MM, Rego ER, Soares WS. Embryogenesis in the anther of different ornamental pepper (Capsicum annuum L.) genotypes. Genet. Mol. Res. 2015; 14: 13349-13363.
Lauxen MS, Kaltachuk-Santos E, Hu CH, Bodanese-Zodanese-Zanettini MH. Association between floral bud size and developmental stage in soybean microspores: implications for anther culture. Braz. Arch. Biol. Technol. 2003; 46(4): 515-520.
Nurhidaya T, Horn R, Rocher T, Friedt W. High regeneration rates in anter culture of intersepcific sunflower hybrids. Plant. Cell. Rep. 1996; 16: 167-173.
Bohorova NE, Antanassov AI. Sunflower (Helianthus annuus L.) in vitro production of haploids. Biotech. In: Bajaj, Y.P.S, editor. Haploids in crop improvement I. Springer verlag, Berlin: 1990; 428-441.
Andronic L, Smerea S, Macovei E. In vitro responses of anthers from different barley genotypes in order to obtain haploid plants. Rom. Agric Res. 2015; 32: 1-9.