Batch butanol fermentation from sugarcane molasses integrated with a gas stripping system: Effects of sparger types and gas flow rates

Main Article Content

Thanawat Thanapornsin
Patthranit Sanchanda
Lakkana Laopaiboon
Pattana Laopaiboon

Abstract

High butanol levels inhibit microbial metabolism and acetone-butanol-ethanol (ABE) fermentation. Gas stripping
is a simple technique to separate solvents from a fermentation broth, which can improve butanol production during
fermentation. Sparger types in gas stripping systems may affect the liquid-gas mass transfer. The aim of this study
was to investigate the effects of sparger types (porous, ring and nozzle designs) and gas flow rates in a gas stripping
system on butanol production from sugarcane molasses by Clostridium beijerinckii TISTR1461 in a batch
fermentation. The gas stripping was started after 24 h of the fermentation, and the gas hold- up values were used
to calculate the liquid-gas mass transfer for the gas stripping system. Results showed that a maximum cumulative
butanol concentration (15.33 g/L) and butanol productivity (0.21 g/L.h) were achieved using a ring sparger at a
gas flow rate of 1. 0 L/min. Under these conditions, the gas hold-up value ( 0. 010) was maximal. The lowest
cumulative butanol concentration (13.17 g/L) and butanol productivity (0.18 g/L.h) were obtained using a nozzle
sparger, corresponding to a minimal gas hold-up value of 0. 003. In conclusion, the results demonstrated that the
higher the gas hold-up, the better the gas- liquid mass transfer attained. The fermentation using the gas stripping
system increased the butanol concentration by approximately 44% compared to that of a fermentation without gas
stripping.

Downloads

Download data is not yet available.

Article Details

Section
Research Articles

References

[1] Al-Shorgani, N.K.N., Kalil, M.S.,Yusoff, W.M.W., 2011. The effect of different carbon sources on biobutanol production using Clostridium saccharoperbutyl acetonicum N1-4. Biotechnology 10, 280-285.

[2] Rochón, E., Ferrari, M.D.,Lareo, C. Integrated., 2017. ABE fermentation-gas stripping process for enhanced butanol production from sugarcane-sweet sorghum juices. Biomass Bioenergy 98, 153-160.

[3] Lee, S.Y., Park, J.H., Jang, S.H., Nielsen, L.K., Kim, J., Jung, K.S., 2008. Fermentative butanol production by Clostridia. Biotechnology and Bioengineering 101, 209-228.

[4] Green, E.M., 2011. Fermentative production of butanol - the industrial perspective. Current Opinion in Biotechnology 22, 337-343.

[5] Kumar, M.,Gayen, K., 2011. Developments in biobutanol production: New insights. Applied Energy 88, 1999-2012

[6] Office of the cane and sugar board., 2016. Status of sugarcane and sugar in Thailand [WWW Document]. URL https://www.ocsb.go.th/th/cms/detail.php?ID=5351&SystemModuleKey=cuntry. (accessed 11.4.18.).

[7] Wechgama, K., Laopaiboon, L.,Laopaiboon, P., 2014. Butanol production from agricultural raw materials by Clostridium spp. The 2nd TSB International Forum 2014, Bangkok

[8] Wechgama, K., Laopaiboon, L.,Laopaiboon, P., 2017. Enhancement of batch butanol production from sugarcane molasses using nitrogen supplementation integrated with gas stripping for product recovery. Industial Crops and Products 95, 216-226.

[9] Li, L., Ai, H., Zhang, S., Li, S., Liang, Z., Wu, Z.Q., Yang, S. T., Wang, J. F., 2013. Enhanced butanol production by coculture of Clostridium beijerinckii and Clostridium tyrobutyricum. Bioresource Technology 143, 397-404.

[10] Xue, C., Zhao, X. Q., Liu, C. G., Chen, L. J., Bai, F. W., 2013. Prospective and development of butanol as an advanced biofuel. Biotechnology Advances 31, 1575-1584.

[11] Jiang, M., Chen, J.N., He, A.Y. Wu, H., Kong, X. P., Liu, J. L., Yin, C. Y., Chen, W. F., Chen, P., 2014. Enhanced acetone/butanol/ethanol production by Clostridium beijerinckii IB4 using pH control strategy. Process Biochemistry 49, 1238-1244.

[12] Yen, H.W., Lin, S.F.,Yang, I.K., 2012, Use of poly (ether-block-amide) in pervaporation coupling with a fermentor to enhance butanol production in the cultivation of Clostridium acetobutylicum. Journal of Bioscience and Bioengineering 113, 372-377.

[13] Abdehagh, N., Tezel, F.H.,Thibault, J., 2014. Separation techniques in butanol production: Challenges and developments. Biomass and Bioenergy 60, 222-246.

[14] Lu, C., Zhao, J., Yang, S.T., Wei, D., 2012. Fed-batch fermentation for n-butanol production from cassava bagasse hydrolysate in a fibrous bed bioreactor with continuous gas stripping. Bioresource Technology 104, 380-387.

[15] Xue, C., Zhao, J., Lu, C. Yang, S.T., Bai, F., Tang, I. C., 2012. High-titer n-butanol production by Clostridium acetobutylicum JB200 in fed-batch fermentation with intermittent gas stripping. Biotechnology and Bioengineering 109, 2746-2756.

[16] Xue, C., Du, G.Q., Sun, J.X., Chen, L. J., Gao, S. S., Yu, M. L., Yang, S. T., Bai, F. W., 2014. Characterization of gas stripping and its integration with acetone–butanol–ethanol fermentation for high-efficient butanol production and recovery. Biochemical Engineering Journal 83, 55-61.

[17] Truong, K.N.,Blackburn, J.W., 1984. The stripping of organic chemicals in biological treatment processes. Environmental Progress 3, 143-152.

[18] Ezeji, T.C., Qureshi, N., Blaschek, H.P., 2003. Production of acetone, butanol and ethanol by Clostridium beijerinckii BA101 and in situ recovery by gas stripping. World Journal of Microbiology and Biotechnology 19, 595-603.

[19] Ezeji, T.C., Qureshi, N.,Blaschek, H.P., 2005. Continuous butanol fermentation and feed starch retrogradation: butanol fermentation sustainability using Clostridium beijerinckii BA101. Journal of Biotechnology 115, 179-187.

[20] Mackay, D.,Leinonen, P.J., 1975. Rate of evaporation of low-solubility contaminants from water bodies to atmosphere. Environmental Science & Technology 9, 1178-1180.

[21] Doran, P.M., 1995. Bioprocess Engineering Principles. Academic Press, UK

[22] Sirisantimethakom, L., Laopaiboon, L., Sanchanda, P., Chatleudmongkol, J., Laopaiboon, P., 2016. Improvement of butanol production from sweet sorghum juice by Clostridium beijerinckii using an orthogonal array design. Industial Crops and Products 79, 287-294.

[23] Qureshi, N.,Blaschek, H.P., 1999. Butanol recovery from model solution/fermentation broth by pervaporation: evaluation of membrane performance. Biomass and Bioenergy 17, 175-184.

[24] Suwanapong, S., Khongsay, N., Laopaiboon, L., Jaisil, P., Laopaiboon, P., 2013. Dried spent yeast and its hydrolysate as nitrogen supplements for single batch and repeated-batch ethanol Fermentation from sweet sorghum juice. Energies 6, 1618-1631.

[25] Sanchanda, P., Laopaiboon, P.,Laopaiboon, L. Enhancement of butanol production efficiency from sugarcane molasses by low-cost nutrient supplementation. In: ICERE 2015 International Conference on Environment and Renewable Energy; 2015 May 20-21; Vienna

[26] Mecozzi, M., 2005. Estimation of total carbohydrate amount in environmental samples by the phenol–sulphuric acid method assisted by multivariate calibration. Chemometrics and Intelligent Laboratory Systems 79, 84-90.

[27] Areesirisuk, A., Laopaiboon, L., Khongsay, N., Laopaiboon. P., 2010. Improvement of gas chromatographic analysis for organic acids and solvents in acetone-butanolethanol fermentation from sweet sorghum juice. African Journal of Biotechnology 9, 6422-6429.

[28] Ezeji, T.C., Karcher, P.M., Qureshi, N., Blaschek, H.P., 2005. Improving performance of a gas stripping-based recovery system to remove butanol from Clostridium beijerickii fermentation. Bioprocess and Biosystems Engineering 27, 207-214.

[29] Lütke-Eversloh, T.,Bahl, H., 2011. Metabolic engineering of Clostridium acetobutylicum: recent advances to improve butanol production. Current Opinion in Biotechnology 22, 634-647.

[30] Wu, Y.D., Xue, C., Chen, L.J., Bai, F. W., 2013. Effect of zinc supplementation on acetone–butanol–ethanol fermentation by Clostridium acetobutylicum. Journal of Biotechnology 165, 18-21.

[31] Cai, D., Wang, Y., Chen, C., Qin, P., Miao, Q., Zhang, C., Li, P., Tan, T., 2016. Acetone-butanol-ethanol from sweet sorghum juice by an immobilized fermentation-gas stripping integration process. Bioresource Technology 211, 704-710.

[32] Lu, C., Dong, J.,Yang, S.T., 2013. Butanol production from wood pulping hydrolysate in an integrated fermentation-gas stripping process. Bioresource Technology 143, 467-475.

[33] Ezeji, T.C., Qureshi, N.,Blaschek, H.P., 2007. Production of acetone butanol (AB) from liquefied corn starch, a commercial substrate, using Clostridium beijerinckii coupled with product recovery by gas stripping. Journal of Industial Microbiology and Biotechnology 34, 771-777.

[34] Schuster, K.C., van den Heuvel, R., Gutierrez, N.A., Maddox, I. S., 1998. Development of markers for product formation and cell cycle in batch cultivation of Clostridium acetobutylicum ATCC 824. Applied Microbiology and Biotechnology 49(6), 669-676.