Oligosaccharides from rice straw and rice husks produced by glycoside hydrolase family 10 and 11 xylanases

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Alisa Pattarapisitporn
Pannapapol Jaichakan
Wannaporn Klangpetch

Abstract

Rice straw (RS) and rice husks (RH) are the by-products obtained from rice farming, which are the remaining non-starch polysaccharides, called cellulose and hemicellulose. The objectives of this study were to investigate the abilities of the glycoside hydrolase family 10 (GH10) and 11 (GH11) commercial xylanases on the production of oligosaccharides from RS and RH by hydrothermal assisted enzymatic hydrolysis. Firstly, RS and RH were pretreated with acetone/ethanol. Then the pretreated biomass was heated by autoclave at 180 ˚C for 10-30 min. The oligosaccharides content in the RS and RH hydrolysates (HRS and HRH) were analyzed by High Performance Anion Exchange Chromatography (HPAEC-PAD). The results indicated that RS and RH treated for 10 min had shown the highest total oligosaccharides content. After that, the HRS and HRH were hydrolyzed with Ultraflo Max (UM10) and Ultraflo L (UL11), belonged to GH10 and GH11 respectively, under condition at 50 ˚C pH of 6.0 for 0-4 h. The highest sugar-reducing content was found while incubating HRS and HRH for 1 h with the aforementioned xylanases. The sugar-reducing contents of HRS and HRH treated with UM10 increased up to 0.24% and 0.17%, respectively, whereas those treated with UL11 increased up to 0.18% and 0.14%, respectively. The results revealed that HRS and HRH treated with UM10 had mainly consisted of xylobiose, while those treated with UL11 had mainly consisted of xylotriose. This study has suggested the potential of GH10 and GH11 xylanases on Xylooligosaccharide (XOS) production using RS and RH as alternative sources.

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References

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