Effects of lotus (Nelumbo nucifera Gaertn.) stamen extract on growth performance, feed utilization and intestinal morphology of catfish (Clarias gariepinus)
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Abstract
The effects of dietary supplementation of lotus (Nelumbo nucifera Gaertn.) stamen extract (NNSE) on growth performance, feed utilization efficiency and intestinal morphology of Catfish (Clarias gariepinus) were evaluated experimentally in a 7-week feeding trial. Fish with an initial weight of 12.54±0.64 g were completely randomized into 4 groups with 3 replications and reared in circular concrete tanks. Group 1, fish were fed the basal diets and served as the control group. Groups 2-4, fish were fed the diets supplemented with 0.1, 0.5 and 1% of NNSE, respectively. At the end of experiment, it was found that fish fed the diets containing NNSE at different levels significantly increased weight gain, specific growth rate and feed conversion ratio compared to the control (P<0.05). No significant differences were observed in the survival rate and the relative weights of internal organs between the groups (P>0.05). Villi heights and widths, muscularis thickness and goblet cell number in anterior and posterior portion of fish intestines observed under the light microscope were significantly increased in the experimental groups compared to the control group (P<0.05). Additionally, feeding behavior, palatability and feed acceptability did not differ among the groups. The optimal level of NNSE observed was 0.1%. Taken together, these findings reveal that NNSE could be applied in fish feed as a natural feed additive without negative side-effects to improve growth, feed utilization and intestinal morphology in Catfish.
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[2] Citarasu T. Herbal biomedicines: a new opportunity for aquaculture industry. Aquacult Int. 2010 Apr;18(3):403-14.
[3] Talpur AD, Ikhwanuddin M. Dietary effects of garlic (Allium sativum) on haemato-immunological parameters, survival, growth, and disease resistance against Vibrio harveyi infection in Asian sea bass, Lates calcarifer (Bloch). Aquaculture. 2012 Oct 5;364-365:6-12.
[4] Talpur AD. Mentha piperita (Peppermint) as feed additive enhanced growth performance, survival, immune response and disease resistance of Asian seabass, Lates calcarifer (Bloch) against Vibrio harveyi infection. Aquaculture. 2014 Jan 15; 420-421:71-8.
[5] Pieters L, Vlietinck AJ. Bioguided isolation of pharmacologically active plant components, still a valuable strategy for the finding of new lead compounds?. J Ethnopharmacol. 2005 Aug 22;100(1-2):57-60.
[6] Mehta NR, Patani EPPPV, Shah B. Nelumbo nucifera (Lotus): a review on ethnobotany, phytochemistry and pharmacology. Indian J Pharm Biol Res. 2013;1(4):152-67.
[7] Mukherjee PK, Mukherjee D, Maji AK, Rai S, Heinrich M. The sacred lotus (Nelumbo nucifera)-phytochemical and therapeutic profile. J Pharm Pharmacol. 2009 Apr;61(4):407-22.
[8] Sridhar KR, Bhat R. Lotus-a
potential nutraceutical source. J Agri Tech. 2007;3(1):143-55.
[9] Sivagurunathan A, Innocent BX, Lakshmi SM. Immunomodulatory effect of dietary Nelumbo nucifera (lotus) in growth and haematology of Cirrhinus mrigala challenged with Pseudomonas aeruginosa. J Applied Pharm Sci. 2012;2(7): 191-95.
[10] Munglue P. Effects of dietary Nelumbo nucifera (lotus) peduncle extract on growth performance of Nile tilapia (Oreochromis niloticus). Proceedings of the 1st Environment and Natural Resources International
Conference; 2014 Nov 6 – 7; Bangkok, Thailand; 2014. P.279-82.
[11] Munglue P. Effect of Nelumbo nucifera Gaertn. leaf extract on growth performance of Nile tilapia (Oreochromis niloticus). SDU Res J. 2015 Jan-Apr;8(1):45-56.
[12] Couto A, Kortner TM, Penn M, Bakke AM, Krogdahl Å, Oliva- Teles A. Effects of dietary phytosterols and soy saponins on growth, feed utilization efficiency and intestinal integrity of gilthead sea bream (Sparus aurata) juveniles. Aquaculture. 2014 Aug 20;432: 295-303.
[13] Fishery Statistics Analysis and Research Group, Department of Fisheries. Fisheries Statistics of Thailand 2012. No. 9/2014. 2014 Aug;P. 26. Thai.
[14] Pirarat N, Boonananthanasarn S, Krongpong L, Katagiri T, Maita M. Effect of activated charcoal- supplemented diet on growth performance and intestinal morphology of Nile tilapia
(Oreochromis niloticus). Thai J Vet Med. 2015 Mar;45(1):113-9.
[15] Heidarieh M, Mirvaghefi AR, Akbari M, Farahmand H, Sheikhzadeh N, Shahbazfar AA, Behgar M. Effect of dietary
Ergosan on growth performance, digestive enzymes, intestinal histology, hematological parameters and body composition of rainbow trout (Oncorhynchus mykiss). Fish Physiol Biochem. 2012 Aug;38(4):1169-74.
[16] Francis G, Makkar HPS, Becker K. Dietary supplementation with a Quillaja saponin mixture improves growth performance and metabolic efficiency in common carp (Cyprinus carpio L.). Aquaculture. 2002 Jan 3;203 (3-4):311-20. [17] Babahydari SB, Dorafshan S, Paykan F, Soofiani NM, Vahabi MR. The physiological changes, growth performance and whole body composition of common carp, Cyprinus carpio fed on diet containing wood betony, Stachys lavandulifolia extract. J Agr Sci Tech. 2014;16:1565-74.
[18] Turan F, Akyurt I. Effects of red clover extract on growth performance and body composition of African catfish Clarias gariepinus. Fisheries Sci. 2015 Jun 10;71(3):618-20.
[19] Turan F, Gurlek M, Yaglioglu D. Dietary red clover (Trifolium pratense) on growth performance of common carp (Cyprinus carpio). J Anim Vet Adv. 2007;6(12): 1429-33.
[20] Turan F. Improvement of growth performance in tilapia (Oreochromis aureus Linnaeus) by supplementation of red clover (Trifolium pratense) in diets. Isr J Aquacult-Bamid. 2006;58(1-4):34-8.
[21] Zhai S-W, Liu, S-L. Effects of dietary quercetin on growth performance, serum lipids level and body composition of tilapia (Oreochromis niloticus). Ital J Anim Sci. 2013;12(3):e85.
[22] Fang C, Ma M, Ji H, Ren T, Mims SD. Alterations of digestive enzyme activities, intestinal morphology and microbiota in juvenile paddlefish, Polyodon spathula, fed dietary probiotics. Fish Physio
Biochem. 2015 Feb;41(1):91-105.
[23] Becker K, Makkar HPS. Effects of dietary tannic acid and quebracho tannin on growth performance and metabolic rates of common carp (Cyprinus carpio L.). Aquaculture. 1999 May 15;175(3-4):327-35.
[24] Buyukcapar HM, Atalay Aİ, Kamalak A. Growth performance of Nile tilapia (Oreochromis niloticus) fed with diets containing different levels of hydrolysable and condensed tannin. J Agr Sci Tech. 2011 Dec;13(Suppl.):1045-51.
[25] Kunanusorn P, Panthong A, Pittayanurak P, Wanauppathamkul S, Nathasaen N, Reutrakul V. Acute and subchronic oral toxicity studies of Nelumbo nucifera stamens extract in rat. J Ethnopharmacol. 2011 Apr 12;134(3):789-95.