Effects of dietary waterlily (Nymphaea pubescens) stamen extract on growth performance and intestinal morphology of common lowland frog (Rana rugulosa)
Article Sidebar
Published:
Jul 15, 2016
Keywords:
Common lowland frog Rana rugulosa Waterlily Nymphaea pubescens Growth performance Intestinal morphology
Main Article Content
Abstract
The effects of diets containing various levels (0, 1, 3 and 5%) of waterlily (Nymphaea pubescens) stamen extract (NPSE) on growth performance and intestinal morphology were investigated in common lowland frog (Rana rugulosa) with an initial weight of 16.09±0.50 g. After 11 weeks of feeding, growth parameters were evaluated. The results showed that the frogs fed with diets supplemented with 3 and 5% of NPSE exhibited significantly higher weight gain, specific growth rate and feed conversion ratio than frogs fed diets supplemented with 0 and 1% of NPSE (P<0.05). Significant differences were not observed in survival rate, hepatosomatic index and intestinosomatic index among the all diet groups (P>0.05). Villi heights, villi widths and the thickness of intestinal muscle layers in posterior intestine were significantly increased in frogs fed the diets containing NPSE (P<0.05). Observations on histology of anterior intestine found a significant increase in villi heights and circular muscularis thickness in the treated groups compared to the control group (P<0.05). However, villi widths and longitudinal muscularis thickness of anterior intestine did not affect by dietary treatment (P>0.05). Feeding behavior and feed acceptability of the experimental groups were the same as the control group. The optimal levels of NPSE observed in this present study were ranged between 3-5%. Our findings suggest that NPSE can be applied in the diets as a growth promoter in common lowland frog.
Article Details
How to Cite
Kamatit, W., Aoki, S., & Munglue, P. (2016). Effects of dietary waterlily (Nymphaea pubescens) stamen extract on growth performance and intestinal morphology of common lowland frog (Rana rugulosa). Asia-Pacific Journal of Science and Technology, 21(2), 30–41. https://doi.org/10.14456/kkurj.2016.28
Section
Research Articles
References
[1] Fishery Statistics Analysis and Research Group, Department of Fisheries. Fisheries Statistics of Thailand 2012. No. 9/2014. 2014 Aug;P. 50. Thai.
[2] Muanmeangsong P, Aditto S, Suriya P. Production and marketing of frog farming in Mueang District, Loei Provine. Prawarun Agr J. 2014 Jan-June;11(1):65-72. Thai.
[3] Pariyanonth P, Daorerk V. Frog farming in Thailand. In Nambiar KKB, Singh T, (editors). Infofish-Aquatech’94: Proceedings of the International Conference on Aquaculture; 1994 Aug 29-31; Colombo, Sri Lanka;1994. P. 1-13.
[4] Zhang C-X, Huang K-K, Wang L, Song K, Zhang L, Li P. Apparent digestibility coefficients and amino acid availability of common protein ingredients in the diets of bull frog, Rana (Lithobates) catesbeiana. Aquaculture. 2015 Feb 1;437; 38-45.
[5] Citarasu T, Herbal biomedicines:
a new opportunity for aquaculture industry. Aquacult Int. 2010 Apr;18(3):403-14.
[6] Durmic Z, Blache D. Bioactive plants and plant products: effects on animal function, health and welfare. Anim Feed Sci Tech. 2012 Sep 21;176(1-4):150-62.
[7] Reverter M, Bontemps N, Lecchini D, Banaigs B, Sasal P. Use of plant extracts in fish aquaculture as an alternative to chemotherapy: current status and future
perspectives. Aquaculture. 2014 Sept 20;433:50-61.
[8] Mohan Maruga Raja MK, Sethiya NK, Mishra SH. A comprehensive review on Nymphaea stellata: a traditionally used bitter. J Adv Pharm Tech Res. 2010 Jun-Sep;1(3):311-19.
[9] Parimala M, Shoba FG. Phytochemical analysis and in vitro antioxidant activity of hydroalcoholic seed extract of Nymphaea nouchali Burm. f. Asian Pac J Trop Biomed. 2013 Nov;3(11):887-95.
[10] Debnath S, Ghosh S, Hazra B. Inhibitory effect of Nymphaea pubescens Willd. flower extract on carrageenan-induced inflammation and CCl4-induced hepatotoxicity in rats. Food Chem Toxicol. 2013 Sep;59:485-91.
[11] Rajagopal K, Sasikala K. Antihyperglycaemic and antihyperlipidaemic effects of Nymphaea stellata in alloxan- induced diabetic rats. Singapore Med J. 2008 Feb;49(2):137-41.40 KKU Res. J. 2016; 21(2)
[12] Antonisamy P, Subash-Babu P, Alshatwi AA, Aravinthan A, Ignacimuthu S, Choi KC, Kim J-H. Gastroprotective effect of nymphayol isolated from Nymphaea stellata (Willd.) flowers: contribution of antioxidant, anti- inflammatory and anti-apoptotic activities. Chem-Biol Interact. 2014 Dec 5;224:157-63.
[13] Fossen T, Larsen A, Kiremire BT, Andersen ØM, Flavonoids from blue flowers of Nymphaea caerulea. Phytochemistry. 1999 Aug;51(8):1133-31.
[14] Sivani G, Reddy DC, Bhaskar M, Effect of Nymphaea meal incorporated diets on growth, feed efficiency and body composition in fingerlings of Cyprinus carpio L. J App Nat Sci. 2013;5(1):5-9.
[15] Pharadee K, Seemabut S, Wiriyakarun S, Munglue P. Effect of Nymphaea pubescens leaf extract on growth of Nile tilapia (Oreochromis niloticus). Local Development toward ASEAN: Engagement University: Proceedings of the 8th Ubon Ratchathani University Conference; 2014 Jul 17-18; Ubon Ratchathani, Thailand; 2014. P. 9-16.
[16] Maneeprasert P, Srisuriyathada A, Wongsathein D, Khattiya R, Pikulkaew S. Effect of cysteamine on growth performance in juvenile red tilapia (Oreochromis sp.). Chiang Mai Vet J. 2009 Apr;7(1): 31-8. Thai.
[17] Choshasee T, Barnette P, Jintasthaporn O, Silapachai W, Junprasirt S. Effect of indian penny wort (Centella asiatica) and phlai (Zingiber montanum) supplementation in feed on growth performance and health of common lowland frog (Rana rugulosa). Proceedings of 48th Kasetsart University Annual Conference: Fisheries; 2010 Feb 3-5; Bangkok, Thailand; 2010. P. 336-44. Thai.
[18] Klahan R, Sirithanawong B. In vitro protein digestibility of bromelain from pineapple crown and enzyme from gastrointestinal tract of common lowland frog. Khon Kaen Arg J. 2015;43(Suppl 1):523-28. Thai.
[19] Kaewtapee V, Wadwijid P, Smitasiri Y. Induction of frog tadpole development with Pueraria mirifica dried tuber and Butea superba root powder. Khon Kaen Arg J. 2011;39(Suppl 1):260-64. Thai.
[20] Suriya N, Promya J, Chitmanat C, Effects of the Spirulina platensis and Phyllanthus emblica Linn. extract additional diets on the reproductive maturation of lowland frog (Rana rugulosa, Wiegmann) and its tadpole development. KKU Res J. 2014 Nov-Dec;19(6):753-62. Thai.
[21] Becker K, Makkar HPS. Effects of dietary tannic acid and quebracho tannin on growth performance and metabolic rates of common carp (Cyprinus carpio L.). Aquaculture. 1999 May 15;175(3-4):327-35.
[22] Haibin H, Yanjiao Z, Kangsen M, Qinghui A, Wei X, Wenbing Z, Yanxian L, Jintao L. Effects of
dietary stachyose on growth performance, digestive enzyme activities and intestinal morphology of juvenile turbot (Scophthalmus maximus L). J Ocean Univ China. 2015 Oct;14(5):905-12.
[23] Hlophe SN, Moyo NAG. Replacing fishmeal with kikuyu grass and moringa leaves: effects on growth, protein digestibility, histological and haematological parameters in Clarias gariepinus. Turk J Fish Aquat Sci. 2014 Sep;14(3): 795-806.
[24] Hlophe SN, Moyo NAG. A comparative study on the use of Pennisetum clandestinum and Moringa oleifera as protein sources in the diet of the herbivorous Tilapia rendalli. Aquacult Int. 2014 Aug;22(4):1245-62.
[25] Antushevich H, Krawczynska A, Kapica M, Herman AP, Zabielski R. Effect of apelin on mitosis,
apoptosis and DNA repair enzyme OGG 1/2 expression in intestinal cell lines IEC-6 and Caco-2. Folia Histochem Cytobiol. 2014;52(1): 51-9.
[26] Zheng Q, Wu Y, Xu H. Effect of dietary oxidized konjac glucomannan on Schizothorax prenanti growth performance, body composition, intestinal morphology and intestinal microflora. Fish Physiol Biochem. 2015 Jun;41(3):733-43.
[27] Fang C, Ma M, Ji H, Ren T, Mims SD. Alterations of digestive enzyme activities, intestinal morphology and microbiota in juvenile paddlefish, Polyodon spathula, fed dietary probiotics. Fish Physiol Biochem. 2015 Feb;41(1):91-105.
[2] Muanmeangsong P, Aditto S, Suriya P. Production and marketing of frog farming in Mueang District, Loei Provine. Prawarun Agr J. 2014 Jan-June;11(1):65-72. Thai.
[3] Pariyanonth P, Daorerk V. Frog farming in Thailand. In Nambiar KKB, Singh T, (editors). Infofish-Aquatech’94: Proceedings of the International Conference on Aquaculture; 1994 Aug 29-31; Colombo, Sri Lanka;1994. P. 1-13.
[4] Zhang C-X, Huang K-K, Wang L, Song K, Zhang L, Li P. Apparent digestibility coefficients and amino acid availability of common protein ingredients in the diets of bull frog, Rana (Lithobates) catesbeiana. Aquaculture. 2015 Feb 1;437; 38-45.
[5] Citarasu T, Herbal biomedicines:
a new opportunity for aquaculture industry. Aquacult Int. 2010 Apr;18(3):403-14.
[6] Durmic Z, Blache D. Bioactive plants and plant products: effects on animal function, health and welfare. Anim Feed Sci Tech. 2012 Sep 21;176(1-4):150-62.
[7] Reverter M, Bontemps N, Lecchini D, Banaigs B, Sasal P. Use of plant extracts in fish aquaculture as an alternative to chemotherapy: current status and future
perspectives. Aquaculture. 2014 Sept 20;433:50-61.
[8] Mohan Maruga Raja MK, Sethiya NK, Mishra SH. A comprehensive review on Nymphaea stellata: a traditionally used bitter. J Adv Pharm Tech Res. 2010 Jun-Sep;1(3):311-19.
[9] Parimala M, Shoba FG. Phytochemical analysis and in vitro antioxidant activity of hydroalcoholic seed extract of Nymphaea nouchali Burm. f. Asian Pac J Trop Biomed. 2013 Nov;3(11):887-95.
[10] Debnath S, Ghosh S, Hazra B. Inhibitory effect of Nymphaea pubescens Willd. flower extract on carrageenan-induced inflammation and CCl4-induced hepatotoxicity in rats. Food Chem Toxicol. 2013 Sep;59:485-91.
[11] Rajagopal K, Sasikala K. Antihyperglycaemic and antihyperlipidaemic effects of Nymphaea stellata in alloxan- induced diabetic rats. Singapore Med J. 2008 Feb;49(2):137-41.40 KKU Res. J. 2016; 21(2)
[12] Antonisamy P, Subash-Babu P, Alshatwi AA, Aravinthan A, Ignacimuthu S, Choi KC, Kim J-H. Gastroprotective effect of nymphayol isolated from Nymphaea stellata (Willd.) flowers: contribution of antioxidant, anti- inflammatory and anti-apoptotic activities. Chem-Biol Interact. 2014 Dec 5;224:157-63.
[13] Fossen T, Larsen A, Kiremire BT, Andersen ØM, Flavonoids from blue flowers of Nymphaea caerulea. Phytochemistry. 1999 Aug;51(8):1133-31.
[14] Sivani G, Reddy DC, Bhaskar M, Effect of Nymphaea meal incorporated diets on growth, feed efficiency and body composition in fingerlings of Cyprinus carpio L. J App Nat Sci. 2013;5(1):5-9.
[15] Pharadee K, Seemabut S, Wiriyakarun S, Munglue P. Effect of Nymphaea pubescens leaf extract on growth of Nile tilapia (Oreochromis niloticus). Local Development toward ASEAN: Engagement University: Proceedings of the 8th Ubon Ratchathani University Conference; 2014 Jul 17-18; Ubon Ratchathani, Thailand; 2014. P. 9-16.
[16] Maneeprasert P, Srisuriyathada A, Wongsathein D, Khattiya R, Pikulkaew S. Effect of cysteamine on growth performance in juvenile red tilapia (Oreochromis sp.). Chiang Mai Vet J. 2009 Apr;7(1): 31-8. Thai.
[17] Choshasee T, Barnette P, Jintasthaporn O, Silapachai W, Junprasirt S. Effect of indian penny wort (Centella asiatica) and phlai (Zingiber montanum) supplementation in feed on growth performance and health of common lowland frog (Rana rugulosa). Proceedings of 48th Kasetsart University Annual Conference: Fisheries; 2010 Feb 3-5; Bangkok, Thailand; 2010. P. 336-44. Thai.
[18] Klahan R, Sirithanawong B. In vitro protein digestibility of bromelain from pineapple crown and enzyme from gastrointestinal tract of common lowland frog. Khon Kaen Arg J. 2015;43(Suppl 1):523-28. Thai.
[19] Kaewtapee V, Wadwijid P, Smitasiri Y. Induction of frog tadpole development with Pueraria mirifica dried tuber and Butea superba root powder. Khon Kaen Arg J. 2011;39(Suppl 1):260-64. Thai.
[20] Suriya N, Promya J, Chitmanat C, Effects of the Spirulina platensis and Phyllanthus emblica Linn. extract additional diets on the reproductive maturation of lowland frog (Rana rugulosa, Wiegmann) and its tadpole development. KKU Res J. 2014 Nov-Dec;19(6):753-62. Thai.
[21] Becker K, Makkar HPS. Effects of dietary tannic acid and quebracho tannin on growth performance and metabolic rates of common carp (Cyprinus carpio L.). Aquaculture. 1999 May 15;175(3-4):327-35.
[22] Haibin H, Yanjiao Z, Kangsen M, Qinghui A, Wei X, Wenbing Z, Yanxian L, Jintao L. Effects of
dietary stachyose on growth performance, digestive enzyme activities and intestinal morphology of juvenile turbot (Scophthalmus maximus L). J Ocean Univ China. 2015 Oct;14(5):905-12.
[23] Hlophe SN, Moyo NAG. Replacing fishmeal with kikuyu grass and moringa leaves: effects on growth, protein digestibility, histological and haematological parameters in Clarias gariepinus. Turk J Fish Aquat Sci. 2014 Sep;14(3): 795-806.
[24] Hlophe SN, Moyo NAG. A comparative study on the use of Pennisetum clandestinum and Moringa oleifera as protein sources in the diet of the herbivorous Tilapia rendalli. Aquacult Int. 2014 Aug;22(4):1245-62.
[25] Antushevich H, Krawczynska A, Kapica M, Herman AP, Zabielski R. Effect of apelin on mitosis,
apoptosis and DNA repair enzyme OGG 1/2 expression in intestinal cell lines IEC-6 and Caco-2. Folia Histochem Cytobiol. 2014;52(1): 51-9.
[26] Zheng Q, Wu Y, Xu H. Effect of dietary oxidized konjac glucomannan on Schizothorax prenanti growth performance, body composition, intestinal morphology and intestinal microflora. Fish Physiol Biochem. 2015 Jun;41(3):733-43.
[27] Fang C, Ma M, Ji H, Ren T, Mims SD. Alterations of digestive enzyme activities, intestinal morphology and microbiota in juvenile paddlefish, Polyodon spathula, fed dietary probiotics. Fish Physiol Biochem. 2015 Feb;41(1):91-105.