Screening of fungi producing ligninolytic enzymes
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Published:
Jul 15, 2016
Keywords:
Phenol red Azure B and RBBR Decolorization Fungi
Main Article Content
Abstract
A total of 61 fungi isolate were subjected for screening of ligninolytic enzyme by dye decolorization plate test. The results presented the diversity of 8 orders belonging to 17 families, 30 genera and 25 unidentified cultures. Seven families of Agaricales, three families of Polyporales, two families of Russulales and one family represent for each of the orders, Boletales, Cantharellales, Phallales, Tremellales and Xylariales. The plate test perceived seven categorical groups as: strains decolorizing a single dye implies for secreting only one of the three main enzymes ; a) Phenol red for LiP, b) Azure B for MnP c) RBBR for Lacc; strains decolorizing two of the organic colors relays for secreting only two enzymes as; d) Phenol red and Azure B, e) Phenol red and RBBR, f) Azure B and RBBR; and g) strains which decolorize three organic dyes produce three of the ligninolytic enzymes.
Article Details
How to Cite
Ghebreslasie, Z., Premjet, D., & Permjet, S. (2016). Screening of fungi producing ligninolytic enzymes. Asia-Pacific Journal of Science and Technology, 21(2), 200–209. https://doi.org/10.14456/kkurj.2016.39
Section
Research Articles
References
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[3] Ali Hazrat. Biodegradation of synthetic Dyes, a Review. J. of Water Air soil pollutant, 2010; 213, 251-273.
[4] Bhattacharyya C. & Banerjee R. Environmental biotechnology: Oxford university press; 2007.
[5] Borokhov O. & Rothenburger S. Rapid Dye Decolorization Method for Screening Potential Wood Preservatives. J of Appl. Environ. Micobiol. 2000; 66(12), 5457–5459.
[6] Casieri L., Anastasi A., & Prigione V. Survey of ectomycorrhizal, litter-degrading, and wood- degrading Basidiomycetes for dye decolorization and ligninolytic enzyme activity. Antonie van Leeuwenhoe. 2010; 98(4), 483.
[7] Deveci T., Unyayar A., & Mazmanci A. Production of Remazol Brilliant Blue R decolourising oxygenase from the culture filtrate of Funalia trogii ATCC 200800. J. of Mol. Catalysis B: Enzymatic. 2004: 30(1), 25-32.
[8] Dhouib, A., Hamza, M., Zouari, H., Mechichi, T., Hmidi, R., Labat, M., . . . Sayadi, S. (2005). Screening for Ligninolytic Enzyme Production by Diverse Fungi from Tunisia. World Journal of Microbiology and
Biotechnology, 21(8-9), 1415-1423.
[9] Diano, N. et al. Non- isothermal bioreactors in enzymatic biodegradation of water polluted by endocrine disruptors: BPA as a model of pollutant. Applied Catalysis B: Environmental. 2007;69: 252-261.
[10] Djarwanto, & Tachibana, S. (2009). Screening of Fungi Capable of Degrading Lignocellulose from Plantation Forests. . Pakistan Journal of Biological Sciences,, 12: 669-675.
[11] Field A., Jong D., Costa F., & Bont D. Biodegradation of Polycyclic Aromatic Hydrocarbons by new Isolates of White Rot Fungi. Appl. and environ. Microbiology. 1992; 2219-2226. (12) Haritash A.K. and Kaushik C.P. Biodegradation aspects of polycyclic aromatic hydrocarbons (PAHs): a review. Journal of hazardous materials,. 2009; 169 (1-3): 1-15.
[13] Herna´ndez-Luna, E., Gutie´ rrez-Soto, & Salcedo-Martinez. Screening for decolorizing
basidiomycetes in Mexico: Screening and selection of ligninolytic basidiomycetes with decolorizing ability in Northeast Mexico. World j. of micro.biotech. 2007; 28, 465-473.
[14] Ja’afaru, M. I. (2013). Screening of Fungi Isolated from Environmental Samples for Xylanase and Cellulase Production. ISRN Microbiology, 2013, 283423. (15) Kalmls E., Yasa D., Kalyoncu F., Pazarbas B., & Kobyigit A.
Ligninolytic enzyme activities in mycelium of some wild and commercial mushrooms. African J. of Biotechno. 7 (23), 4314-4320.
[16] Kiiskinen L., Ratto M., & Kruus K. (2004). Screening for novel laccase-producing microbes. J. of Appl. Microbiol. 2008; 97(3), 640-646.
[17] Knapp S., Newby S., & Reece, P. (). Decolorization of dyes by wood-rotting basidiomycete fungi. J. of Enzyme and Microbial Techno. 1995; 17(7), 664-668.
[18] Kuhad, R.C., A.S., 2007. Ligninocellulose Biotechnology Future Prospects. I.K.International publishing house Pvt. [19] Kumari B., Upadhyay C., & Atri S. Screening and Evaluation of Extra-Cellular Oxidases in Some Termitophilous and Lepiotoid Mushrooms. W. J. of Agric. Sci. 2012; 8(4), p409.