Production of fumaric acid from oil palm empty fruit bunch

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Published: Jul 15, 2016
DOI: https://doi.org/10.14456/kkurj.2016.36
Keywords:
Fumaric acid oil palm empty fruit bunch isolation and screening separate hydrolysis and fermentation (SHF)

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Panida U-thai
Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Chatuchak, Bangkok 10900, Thailand
Pilanee Vaithanomsat
Enzyme Technology and Waste Management Research Unit, Kasetsart Agricultural and Agro-Industrial Product Improvement Institute (KAPI), Kasetsart University, Chatuchak, Bangkok, 10900, Thailand
Antika Boondaeng
Enzyme Technology and Waste Management Research Unit, Kasetsart Agricultural and Agro-Industrial Product Improvement Institute (KAPI), Kasetsart University, Chatuchak, Bangkok, 10900, Thailand
Anfal Talek
Enzyme Technology and Waste Management Research Unit, Kasetsart Agricultural and Agro-Industrial Product Improvement Institute (KAPI), Kasetsart University, Chatuchak, Bangkok, 10900, Thailand
Sawitri Chuntranuluck
Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Chatuchak, Bangkok 10900, Thailand

Abstract

Oil palm empty fruit bunch (EFB), one of the most abundant agricultural residues in Thailand, is an attractive lignocellulosic material for value added agricultural products. They could be used as a raw material for cheap renewable feedstock to produce acids and many other value-added products. This research is to investigate the potential of EFB as a raw material for fumaric acid production through separate hydrolysis and fermentation (SHF) by the selected fungal isolate K20. Steam explosion was used in this study to pretreat the lignocellulosic material. A Cellic CTec2 enzyme (25 FPU/ml) was used to hydrolyze the pretreated material into glucose. Response surface methodology (RSM) based on
central composite design (CCD) was applied to optimize the effect of the medium composition including solid loading, (NH4)2SO4 and Na2CO3 on fumaric acid concentration. The optimized medium consisted of (g/L): solid loading 166.70, (NH4)2SO4 0.29 and Na 2CO3 3.07. The maximum predicted value of fumaric acid concentration of 5.18 g/L was obtained, which corresponded with the observed values of 5.30 g/L

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How to Cite
U-thai, P., Vaithanomsat, P., Boondaeng, A., Talek, A., & Chuntranuluck, S. (2016). Production of fumaric acid from oil palm empty fruit bunch. Asia-Pacific Journal of Science and Technology, 21(2), 221–228. https://doi.org/10.14456/kkurj.2016.36
Issue
Vol. 21 No. 2 (2016): June
Section
Research Articles

References

[1] Mosier, N., C. Wyman, B. Dale, R. Elander, Y.Y. Lee, M. Holtzapple and M. Ladish. 2005. Features of promising technologies for pretreatment of lignocellulosic biomass. Bioresour. Technol. 96: 673-686.
[2] Roa Engel, C.A., Straathof, A.J.J., Zijlmans, T.W., van Gulik, W.M., van der Wielen, L.A., 2008. Fumaric acid production by fermentation. Appl. Microbiol. Biotechnol. 78(3), 379–389.
[3] Zhou, Z., Du, G., Hua, Z., Zhou, J., Chen, J., 2011. Optimization of fumaric acid production by Rhizopus delemar based on the morphology formation. Bioresour. Technol. 102 (20), 9345–9349.
[4] Roa Engel, C.A., van Gulik, W.M., Marang, L., van der Wielen, L.A.M., Straathof, A.J.J., 2011. Development of a low pH fermentation strategy for fumaric acid production by Rhizopus oryzae. Enzyme Microb. Technol. 48 (1), 39–47
[5] Gangl, I.C., Weigand, W.A., Keller, F.A., 1990. Economic comparison of calcium fumarate and sodium fumarate production by Rhizopus arrhizus. Appl.Biochem. Biotechnol. 24 (1 ), 663–677.
[6] Huang, C.F., Lin, T.H., Guo, G.L., Hwang, W.S., 2009. Enhanced ethanol production by fermentation of rice straw hydrolysate without detoxification using a newly adapted strain of Pichia stipitis. Bioresour. Technol. 100 (17), 3914–3920.