Biohydrogen production by microalgae isolated from the rice paddle field in Thailand
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Abstract
Hydrogen produced by cyanobacteria and green algae is a very interesting energy carrier because it is produced by a photosynthetic pathway using sunlight as an energy source. In this study, 59 cyanobacterial and green algal strains were isolated from soil and water sources of rice paddle field in Thailand. Out of them, 9 cyanobacterial isolates and 9 green algal isolates were purified. Among them, unicellular cyanobacterial isolate AngS1 showed the highest H2 production rate. Its highest H2 production rate of 389.630±72.084 nmolH 2 mg chl-1 h-1 was found in cells grown in BG11 for 1 week followed by incubating cells in BG11 0 for 24 hours and adaptation under dark anaerobic condition for 2 hours. The optimal concentrations of glucose, MgSO4.7H2O and Fe3+ for H2 production rate were 0.189 mmolC L-1, 3 mM, and 20 μM, respectively. The highest H2 accumulation of 4,174.364±278.324 nmolH 2 mg chl-1 was obtained when incubating cells in the optimal medium for 11 days.
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