Hydrogen production by unicellular green alga chlorella sp. LSD-W2 isolated from seawater in Thailand
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Published:
Jul 15, 2016
Keywords:
Hydrogen production marine green algae heterotrophic cultivation
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Abstract
Green algae are able to convert the unlimited sunlight energy to produce hydrogen via photosynthesis. In seawater, several kinds of marine microalgae are widespread and abundant and have been shown to tolerate and survive under the extreme salt concentrations. This work aimed to study the screening of high H2 producing marine green algal strains isolated from the Gulf of Thailand and the Andaman Sea, and the selection of the highest H2 producing strain. Its H2 production was investigated under photoheterotrophic cultivation. The result revealed that among 20 marine green algal strains, the green alga Chlorella sp. LSD-W2 gave the highest H2 production rate in both light and dark anaerobic conditions. During photoheterotrophic cultivation Chlorella sp. LSD-W2 was rapidly grown in TAP (Tris-Acetate-Phosphate) medium and reached the stationary growth phase after 36 h of cultivation. The highest photohydrogen production rate was found in cells incubated in NH 4Cl-deprived TAP medium. It was approximately 20-fold higher than H2 production rate of cells in a normal TAP medium.
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How to Cite
Tinpranee, N., Incharoensakdi, A., & Phunpruch, S. (2016). Hydrogen production by unicellular green alga chlorella sp. LSD-W2 isolated from seawater in Thailand. Asia-Pacific Journal of Science and Technology, 21(2), 256–266. https://doi.org/10.14456/kkurj.2016.32
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Research Articles
References
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[23] Wykoff DD, Davies JP, Melis A, Grossman AR. The regulation of photosynthetic electron transport during nutrient deprivation in Chlamydomonas reinhardtii. Plant Physiol. 1998 May;117(1):129-39.
[24] Miura Y, Ohta S, Mano M, Miyamoto K. Isolation and characterization of a unicellular marine green alga exhibiting high activity in dark hydrogen production. Agric Biol Chem. Jun 2;50(11),2837-44. (25) Guan Y, Deng M, Yu X, Zhang W. Two-stage photo-biological production of hydrogen by marine green alga Platymonas subcordiformis. Biochem Eng J. 2004 Jul;19(1-19):69-73.
[2] Yu J, Takahashi P. Biophotolysis-based hydrogen production by cyanobacteria and green algae. Communicating current research and educational topics and trends in applied microbiology. A. Méndez-Vilas (Ed). 2007.
[3] Tsygankov AA, Kosourov SN, Tolstygina IV, Ghirardi ML, Seibert M. Hydrogen photoproduction by sulfur-deprived Chlamydomonas reinhardtii under photoautotrophic conditions. Int J Hydrogen Energy. 2006 Jun 24;3(11):1574-84.
[4] He M, Li L, Zhang L, Liu J. The enhancement of hydrogen photo production in Chlorella protothecoides exposed to nitrogen limitation and sulfur deprivation. Int J Hydrogen Energy. 2012 Aug 12;37(22):16903-15.
[5] Abeliovich A, Weisman D. Role of heterotrophic nutrition in growth of the alga Scenedesmus obliquus in high-rate oxidation ponds. Appl Environ Microbiol.1978 Jan;35(1):32-7.
[6] Happe T, Naber, JD. Isolation, characterization and N-terminal amino acid sequenceof hydrogenase from the green alga Chlamydomonas reinhardtii. Eur J Biochem. 1993 Jun;214(2):475-81.
[7] Rattana S, Incharoensakdi A, Phunpruch S. Hydrogen production of a unicellular green alga Chlorella vulgaris var. vulgaris TISTR8621. In: The 3rd International conference on fermentation technology for value added agricultural products and the 2009 Asian bio-hydrogen symposium; 2009 August 26-28; Khonkaen, Thailand. Hy3p1
265KKU Res. J. 2016; 21(2)
[8] Maneeruttanarungroj C, Lindblad P, Incharoensakdi A. A newly isolated green alga, Tetraspora sp. CU2551, from Thailand with efficient hydrogen production. Int J Hydrogen Energy.2010 Aug9;35(24):13193-9.
[9] Rattana S, Junyapoon S, Incharoensakdi A, Phunpruch S. Hydrogen production by the green alga Scenedesmus sp. KMITL-O1 under heterotrophic conditions. Proceedings of the 8th international symposium on biocontrol and
biotechnology; 2010October 4-6; Pattaya, Chonburi Province, Thailand. P. 114-20.
[10] Rameshprabu R, Kawaree R, Unpaprom Y. A newly isolated green alga, Pediastrum duplex Meyen, from Thailand with efficient hydrogen production.Int J Sust Green Energy. 2015Apr1;4 (1-1):7-12.
[11] Philipps G, Happe T, Hemschemeier A. Nitrogen deprivation results in photosynthetic hydrogen production in Chlamydomonas reinhardtii. Planta. 2012 Apr;235(4):729-45.
[12] Batyrova KA, Gavrisheva A, Ivanova E, Liu J, Tsygankov A. Sustainable hydrogen photoproduction by phosphorus- deprived marine green microalgae Chlorella sp. Int J MolSci. 2015 Jan 26;16(2):2705-16.
[13] Batyrova KA, Tsygankov A, Kosourov S. Sustained hydrogen photoproduction by phosphorus deprived Chlamydomonas reinhardtii cultures. Int J Hydrogen Energy. 2012 Jan 6; 37(10): 8834-9.
[14] Hoshaw RW, Rosowksi JR. Methods for microscopic algae. In Handbook of Phycological Methods. Culture Methods and Growth Measurements (Stein, J., editor), 1973 53-67. Cambridge University Press, New York.
[15] Rippka R, Deruelles J, Waterbury JB, Herdman M, Stanier RY. Generic assignments, strain
histories and properties of pure cultures of cyanobacteria. J Gen Microbiol. 1979 Feb15;111(2): 1-61.
[16] Harris EH. The Chlamydomonas source book: a comprehensive guide to biology and laboratory use. San Diego: Academic Press; 1989.
[17] Lee YK, Shen H, Basic culturing techniques. In Handbook of microalgal culture (Richmond, A., editor), 2004 IS Press
[18] Allahverdiyeva Y, Leino H, Saari L, Fewer DP, Shunmugam S, Sivonen K, Aro EM. Screening for biohydrogen production by cyanobacteria isolated from the Baltic Sea and Finnish lakes. Int J Hydrogen Energy. 2009 Dec 30; 35(3):1117-27.
[19] Ghirardi ML, Zhang L, Lee JW, Flynn T, Seibert M, Greenbaum E, Melis A. Microalgae: a green source of renewable H2. Trends Biotechnol. 2000 Dec 1;18(12): 506-11.
[20] Ghirardi ML, Posewitz MC, Maness PC, Dubini A, Yu J, Seibert M. Hydrogenases and hydrogen photoproduction in oxygenic photosynthetic organisms. Annu Rev Plant Biol. 2006 Dec 6;58: 71-91.
266 KKU Res. J. 2016; 21(2)
[21] Winkler M, Hemschemerier A, Gotor C, Melis A, Happe T. [Fe]- hydrogenase in green algae: photo-fermentation and hydrogen evolution under sulfur deprivation. Int J Hydrogen Energy. 2002 Nov-Dec;27(11-12):1431-9.
[22] Wu SX, Huang R, Xu LL, Yan GG, Wang QX. Improved hydrogen production with expression of hemH and lba genes in chloroplast of Chlamydomonas reinhardtii. J Biotechnol. 2010 Apr 1;146(3): 120-5.
[23] Wykoff DD, Davies JP, Melis A, Grossman AR. The regulation of photosynthetic electron transport during nutrient deprivation in Chlamydomonas reinhardtii. Plant Physiol. 1998 May;117(1):129-39.
[24] Miura Y, Ohta S, Mano M, Miyamoto K. Isolation and characterization of a unicellular marine green alga exhibiting high activity in dark hydrogen production. Agric Biol Chem. Jun 2;50(11),2837-44. (25) Guan Y, Deng M, Yu X, Zhang W. Two-stage photo-biological production of hydrogen by marine green alga Platymonas subcordiformis. Biochem Eng J. 2004 Jul;19(1-19):69-73.