Ethanol production from pineapple waste by co-culture of saccharomyces cerevisiae TISTR 5339 and candida shehatae KCCM 11422
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Published:
Jul 15, 2016
Keywords:
Ethanol production pineapple waste ultrasonic Saccharomyces cerevisiae TISTR 5339 Candida shehatae KCCM 11422
Main Article Content
Abstract
The ethanol production from alkaline pretreated pineapple waste (core and peel) by using co-culture process of S. cerevisiae TISTR 5339 and C. shehatae KCCM 11422 was evaluated. The potential of ultrasound-assisted alkaline pretreatment was applied to enhance total reducing sugar concentration. The highest yield of total reducing sugar was
obtained at 21.84 g/g dried sample after pretreatment by 2% NaOH with ultrasonic-assistance for 60 min subsequently by enzymatic hydrolysis. The compositions of pretreated pineapple waste were 40.52±0.99% cellulose, 24.03±1.52% hemicellulose and 2.27±0.23% lignin. However, the dry matter loss reached to 44.67±3.21% after pretreatment. The structural changes of pretreated pineapple waste were observed by scanning electron microscopy (SEM). The maximum ethanol concentration and ethanol yield after 24 h fermentation were 9.73±0.15g/L and 0.45±0.01 g/g, respectively.
Article Details
How to Cite
Soontornchaiboon, W., Chunhachart, O., & Pawongrat, R. (2016). Ethanol production from pineapple waste by co-culture of saccharomyces cerevisiae TISTR 5339 and candida shehatae KCCM 11422. Asia-Pacific Journal of Science and Technology, 21(2), 347–355. https://doi.org/10.14456/kkurj.2016.48
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Research Articles
References
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[4] Mcintosh S, Vancov T. Enhanced enzyme saccharification of Sorghum bicolor straw using dilute alkali pretreatment. Bioresour Technol. 2010;101:6718-6727.
[5] Sills DL, Gossett JM. Assessment of commercial hemicellulases for saccharification of alkaline pretreated perennial biomass. Bioresour. Technol. 2011;102: 1389-1398.
[6]) Mosier N, Wyman C, Dale B, Elander R, Lee YY, Holtazapple M, et al. Features of promising technologies for pretreatment of lignocellulosic biomass. Bioresour Technol. 2005;96:673-86.
[7] Madeleine JB, Dongke Z. Effect of ultrasound on lignocellulosic biomass as a pretreatment for b i o r e f i n e r y a n d b i o f u e l applications. Ind Eng Chem Res. 2013;52:3563-80.
[8] Hickert LR, Da Cunha-Pereira F, De Souza-Cruz PB, Rosa CA, Ayub MAZ. Ethanogenic fermentation of cocultures of Candida shehatae HM 52.2 and Saccharomyces cerevisiae ICVD254 in synthetic mediumand rice hull hydrolysate.
Bioresour Technol. 2013;131:508–514.
[9] Van SPJ, Wine RH. Use of detergents in the analysis of fibrous feeds.IV. Determination of plant cell-wall consti-tuents. J Assoc Off Anal Chem. 1967;50:50-55.
[10] Van SPJ, Wine RH. Determination of lignin and cellulose in a c i d - d e t e rg e n t f i b e r w i t h permanganate. J Assoc Off Anal Chem. 1968;51:780-85.
[11] Miller G. Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar. Anal Chem.
1959;31:426-29.
[12] Peerawat N, Saowanit K, Wanwisa N. Utilization of Pineapple Waste as Roughage Source in Goats. Khon
Kaen Agr J. 2011;39:399-412.
[13] Carvalheiro F, Duarte LC, Girio FM. Hemicellulose biorefineries: a review on biomass pretreatment. J Sci Ind Res. 2008;67:849-64.
[14] Zhang Y, Fu E, Liang J. Effect of u l t r a s o n i c w a v e s o n t h e saccharification processes of lignocellulose. Chem Eng Technol. 2008;31:1510-15.
[15] Filson PB, Dawson-Andoh BE. Sono-chemical preparation of cellulose nanocrystals from lignocellulose derived materials. B i o r e s o u r T e c h n o l . 2009;100:2259−64.
[16] Sukri SSM., Rahman RA., Illias RM, Yaakob H. Optimization of alkaline pretreatment condition of oil palm fronds in improving the lignocelluloses contents for reducing sugar production. Roman Biotechnol Lett. 2014;19(1): 9006-17.
[17] Claassen PAM, JB van Lier, AM Lopez Contreras, EWJ van Niel, L Sijtsma, AJM Stams, SS de Vries, RA Weusthuis. Utilisation of biomass for the supply of energy carriers. Appl Microbiol and Biotechnol. 1999;52:741-755.
[18] Tian S, Zang J, Pan Y, Liu J, Yuan Z, Yan Y. Construction of a recombinant yeast strain converting xylose and glucose to ethanol. Front Biol China. 2008;3:165–169.