Production of dextran by leuconostoc mesenteroides TISTR 053 in fed batch fermentation
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Published:
Jul 15, 2016
Keywords:
dextran fed batch fermentation Leuconostoc production
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Abstract
Dextran is a natural homopolysaccharide composed of α(1,6)-linkages in their major chain and is synthesized by dextransucrase of the microbial cell in the presence of sucrose as a substrate. The production of dextran by sucrose fermentation using Leuconostoc mesenteroides TISTR 053 was carried out in a Biostat B plus fermenter with total working volume of 3 liter. The effects of sucrose concentrations and modes of operation (batch and fed batch) were studied. The maximum dextran production obtained after 24 hours of incubation at 37 oC with 20% sucrose in batch fermentation. The fed batch fermentation promoted the dextran productivity. The structure of dextran was determined and confirmed by FTIR and NMR.
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How to Cite
Nuwan, P., Piwpan, P., Jaturapiree, A., & Jaturapiree, P. (2016). Production of dextran by leuconostoc mesenteroides TISTR 053 in fed batch fermentation. Asia-Pacific Journal of Science and Technology, 21(2), 366–375. https://doi.org/10.14456/kkurj.2016.45
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Research Articles
References
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[3] Sidebotham RL. Dextrans. Adv. Carbohydr. Chem. Biochem.1974; 30:371-444.
[4] Monsan P, Bozonnet S, Albenne C, Joucla G, Willemot RM and R e m a u n d - S i m e o n M . Homopolysaccharides from lactic acid bacteria. Int. Dairy J., 2001; 11:675-685.
[5] Koepsell HJ and Tsuchiya HM. Enzymatic synthesis of dextran, J. Bact. 1952; 63: 293–295.
[6] Shamala TR and Prasad MS. Preliminary studies on the production of high and low viscosity dextran by Leuconostoc spp. Process Biochem. 1995; 30: 237–241.
[7] P urama RK and G oyal A . Dextransucrase production by Leuconostoc mesenteroides. Ind. J. Microbiol. 2005; 2: 89-101.
[8] P urama RK and G oyal A . Identification, effective purification and functional characterization of
dextransucrase from Leuconostoc mesenteroides NRRL B-640. Bioresource Technol. 2008; 99: 3635-3642.
[9] Sarwat F, Qader SAU, Aman A and A h m e d N . P r o d u c t i o n & Characterization of a Unique Dextran from an Indigenous Leuconostoc mesenteroides CMG713. Int. J. Biol. Sci. 2008; 4(6):379-386.
[10] Sankpal NV, Joshi AP, Sainkar SR and Kulkarni BD. Production of d e x t r a n b y R h i z o p u s s p . immobilized on porous cellulose support. Process Biochem. 2001; 37: 395–403.
[11] Vedyashkina TA, Revin VV and Gogotov IN. Optimizing the Conditions of Dextran Synthesis by the Bacterium Leuconostoc mesenteroides Grown in a Molasses-Containing Medium. Appl. Biochem. Microb. 2005; 41(4): 361–364.
[12] Yamane T and Shimizu S. Fed-batch techniques in microbial process. Adv. Biochem. Biotechnol. Eng. 1984; 30: 148–194.
[13] DuBois M , Gilles KA, Hamilton JK, Rebers PA and Smith F. Colorimetric method for determination of sugars and related substances.Anal Chem. 1956; 28(3): 350–356.
[14] Kim D, Robyt JF, Lee SY, Lee JH and Kim YM. Dextran molecular size and degree of branching as a function of sucrose concentration, pH and temperature of reaction of Leuconostoc mesenteroides B512FMCM dextransucrase. Carbohydr Res. 2003; 338: 1183-1189.
[15] Tsuchiya HM, Hellman NN, Koepsell HJ, Carman J, Strainger CS, Rogovin SP, Bagard MO, Bryant G, Feger VH, Hoffnan CA, Senti FR and Jackson RW. Factors affecting molecular weight of enzymatic synthesized dextran. J Amer Chem Soc. 1955; 77: 2412-2419.
[16] Shingle KI. Determination of structural peculiarities of dextran, pullulan and c-irradiated pullulan b y F o u r i e r - t r a n s f o r m I R spectroscopy. Carbohydr. Res. 2002; 337: 1445–1451.
[17] Liu C, Lin Q, Gao Y, Ye L, Xing Y and Xi T. Characterization and antitumor activity of polysaccharide from Strongylocentrotus nudus eggs. Carbohydr. Polym. 2007; 67: 313–318.
[18] Purama RK, Goswami P, Khan AT and Goyal A. Structural analysis and properties of dextran produced by Leuconostoc mesentroides NRRL B-640. Carbohydr. Polym. 2009; 76: 30–35.
[19] Ahmed RZ, Siddiqui K, Arman M and Ahmed N. Characterization of high molecular weight dextran produced by Weissella cibaria CMGDEX3 .Carbohydr. Polym. 2012; 90: 441– 446.
[20] Seymour FR. Correlation of the structure of dextran to their 1 H NMR spectra. Carbohydr. Res. 1979a; 74: 77–92.