Isolation and production of polyhydroxybutyrate (PHB) from isolated strain bacillus sp. using crude glycerol as a carbon source

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Published: Jul 15, 2016
DOI: https://doi.org/10.14456/kkurj.2016.44
Keywords:
Bacillus biodiesel crude glycerol polyhydroxybutyrate production

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Phakphimol Piwpan
Department of Biotechnology, Faculty of Engineering and Industrial Technology, Silpakorn University, Nakhon Pathom 73000, Thailand
Adisak Jaturapiree
Department of Chemistry, Faculty of Science and Technology, Nakhon Pathom Rajabhat University, Muang, Nakhon Pathom 73000, Thailand.
Phimchanok Jaturapiree
Department of Biotechnology, Faculty of Engineering and Industrial Technology, Silpakorn University, Nakhon Pathom 73000, Thailand

Abstract

Polyhydroxybutyrate (PHB) is polyester produced by a range of microbes under unfavorable growth conditions and stored as an intracellular carbon and energy material. PHB production is more expensive than petrochemical polymer production. The main production cost is the cost of carbon substrate. The aim of this study is to produce PHB using a cheap carbon source, crude glycerol, which is a major byproduct in the biodiesel manufacturing process. PHB producing bacteria species were isolated from the soil collected around biodiesel plants and screened by Nile Red staining method. The effects of sources and concentrations of crude glycerol on the biosynthesis of PHB were
investigated. The maximum PHB content obtained after 24 hours of batch cultivation was 23.59 % of cell dry weight at initial crude glycerol of 10 g/L and C:N ratio of 1:0.4.

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How to Cite
Piwpan, P., Jaturapiree, A., & Jaturapiree, P. (2016). Isolation and production of polyhydroxybutyrate (PHB) from isolated strain bacillus sp. using crude glycerol as a carbon source. Asia-Pacific Journal of Science and Technology, 21(2), 376–384. https://doi.org/10.14456/kkurj.2016.44
Issue
Vol. 21 No. 2 (2016): June
Section
Research Articles

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