In vitrotissue culture of dendrobium kontumense gagnep

Abstract

Tissue culture of Dendrobium kontumense Gagnep.was performed by culturing shoots (0.5 cm long) which were derived from protocorms on half-Murashige and Skoog ( 1/2  MS) and MS media. The average number of shoots was 1.40 shoot/explant, and the average length of the root was 2.86 cm when cultured on MS medium. When seedlings were transferred to culture on MSmedium supplemented with 0, 0.5, 1, 2, 3, 4 and 5 mg/L Naphthaleneacetic acid (NAA) in combination with 0, 0.5, 1, 2, 3, 4 and 5 mg/L N 6 -Benzyladenine (BA). The average number of shoots (2.80 shoot/explant), the high average length of the shoot (4.02 cm), the average number of roots (8.16 root/shoot) and the average length of the root (15.00 cm), were best achieved on MS medium supplemented with 0.5 mg/L NAA and 0.5 mg/L BA. They were transferred MS medium containing with 0, 5, 10, 15, 20 and 25% coconut water, the best shoot growth was observed with 15% coconut water. Upon subculture, the plantlets on MS medium supplemented with 0, 2, 4, 6, 8 and 10% ground banana, the shoots were formed at the best on MS medium with 6% ground banana. The plantlets transplanted to pots containing coconut hack under natural conditions displayed 90% survival.