Vitro Propagation of Globba marantina L

Abstract

The long divided and undivided bubils of Globba marantina L. were cultured on Murashige and Skoog (MS) medium supplemented with 3 mg/l Benzyladenine (BA) and 0.5 mg/l Naphthaleneacetic acid (NAA) for 8 weeks. The result showed that the long divided bubils produced 20 percent of plant regeneration, and the average number of shoots was 3.5 shoot/explant. Callus induction, shoot and root formation were observed when cultured microshoot 1 cm in length on MS medium with various concentrations of Thidiazuron (TDZ), NAA combined with BA for 8 weeks. The highest percentage of callus induction was 40% when cultured microshoot on MS medium supplemented with 0.5 mg/ l NAA and 3 mg/l BA. The best result for shoot formation was 7.10 shoot/explant achieved on MS medium added with 2 mg/l TDZ. The highest number of root was 25.33 root/explant obtained on medium added with 0.5 mg/l NAA and 0.5 mg/l BA.