DNA figer printing analysis of fourteen species of orchids using ISSR technique

Abstract

The DNA fingerprint can be used for identification the orchid cultivars and also used in orchid breeding in the future. The DNA fingerprint and genetic diversity among 12 Dendrobium, 1 Ascocentrum, and 1 Rhynchostylis orchid cultivars were studied using Inter Simple Sequence Repeat (ISSR) marker technique. The results showed that eighth PCR products which were derived from ten oligonucleotide primers amplification (PP1, PP2, PP3, PP4, PP5, PP6, PP7, PP8, PP9 and PP10). There were three primers; PP3, PP5 and PP7 could generate different bands among fourteen orchid cultivars and contained band size from 186-2159 base pair that amplified from the good quality orchid DNAs in the PCR. Hence these ISSR technique markers not only can be used for identification orchid cultivars but also for genetic database useful for future breeding program.