Solid state production of esterase using groundnut oil cake by fish intestinal isolate bacillus circulans

Abstract

A bacterium that produces esterase was isolated from the gut of marine fish Sardinella longiceps and identified as Bacillus circulans. The esterase production was optimized by using economically cheap agro industrial substrate groundnut oil cake under solid state fermentation. The effect of triglycerides and surfactants on esterase production indicated that the maximum amount of esterase production was observed in castor oil and Tween 80 added medium. The suitable NaCl concentration observed for maximum enzyme production was 4%. The effective nitrogen and carbon sources that enhanced the esterase production were found to be ammonium hydrogen carbonate and maltose. The effect of various kinds of metal ions inferred that, CaCl2 added medium favored the maximum amount of esterase production. The optimum pH, temperature and moisture level for the excellent esterase production were found to be 9, 600 C and 75%, respectively. The effect of various organic solvents revealed that hexane (102%), paraffin liquid (104%) and nonane (107%) were found to increase the esterase production over the control (100%)