Production, characterization and hydrolysation products of xylanase from Bacillus subtilis GN156

Abstract

To improve the production of xylanase from Bacillus subtilis GN156, xylan and corncob powder were tested separately as inducers. By varying the concentrations of both inducers from 0-2 % (w/v), the xylanase production from xylan containing medium was higher than those from corncob powder containing medium in all treatments. The highest xylanase activity of 1.47 U/ml was obtained from the production medium supplement with xylan 1.5 % (w/v). The effects of pH and temperature on the enzyme activity and stability were also studied. Xylanase activity was effective at wide range of pH 4-7 with relative activity more than 75%, while its optimum pH and optimum temperature were shown at 5 and 40o C, respectively. Moreover, this enzyme was stable to the temperature up to 50o C for 30 min with the remaining activity more than 50 %. The hydrolysation products from the action of xylanase were investigated by using xylan as a substrate and determined by the technique of Thin Layer Chromatography (TLC). Xylose and 3 different kinds of xylo-oligosaccharides were detected as the products.