Potential of glucoamylase and cellulase production using mixed culture of Aspergillus niger TISTR 3254 and Trichoderma reesei TISTR 3081

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Published: Apr 20, 2017

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Krongchan Ratanaphadit
epartment of Biotechnology, Faculty of Sciences, Burapha University, Chonburi, Thailand
Kwanjai Kaewjan
epartment of Biotechnology, Faculty of Sciences, Burapha University, Chonburi, Thailand
Somchit Palakas
epartment of Applied Radiation and Isotopes, Faculty of Science, Kasetsart University, Bangkok, Thailand

Abstract

Production of glucoamylase and cellulase using mixed culture of Aspergillus niger TISTR 3254 and Trichoderma reeseiTISTR3081aswellaspotentialofusingcassavabagasseascarbonsourceforenzymesproductionwereinvestigated. Culturemediacomprisingofcassavabagasse(1-3%)ascarbonsourceinsteadofCMCsupplementedwith1%ofselected nitrogensource(ammonium sulphate,ureaor soybean meal) wereused. Experimentsunder submergefermentation conditionwereconductedatroomtemperatureandundershakingspeedof150rpmduring5days. Theresultsshowedthat mixed culture of Aspergillus niger TISTR 3254 and Trichoderma reesei TISTR3081growninculturemediumcontainingof2% cassavabagasse and1% ammonium sulphate exhibitedthehighest enzyme activity. Glucoamylase enzyme activity of7,734±0.84 U ml -1 andcellulolytic enzyme activitiesof5.76±0.09(FPase) and27.96±0.44(CMCase) U ml -1 were obtained.

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How to Cite
Ratanaphadit, K., Kaewjan, K., & Palakas, S. (2017). Potential of glucoamylase and cellulase production using mixed culture of Aspergillus niger TISTR 3254 and Trichoderma reesei TISTR 3081. Asia-Pacific Journal of Science and Technology, 15(9), 833–842. Retrieved from https://so01.tci-thaijo.org/index.php/APST/article/view/84065
Issue
Vol. 15 No. 9 (2010): September
Section
Research Articles