Antioxidant activity and protective effect against oxidative stress induced-hemolysis of Nymphaea lotus L. extracts
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Abstract
Reactive oxygen species have been shown to play a role in degenerative diseases. Water lily or Nymphaea lotus L. is an aquatic plant. It has been used as medicinal plant to treat chronic diseases, especially cardiovascular disease, diabetes mellitus and aging. This aim of this study was to determine the phytochemical composition, antioxidant activities, ferric reducing antioxidant power and protective effect against oxidative stress induced-hemolysis of Nymphaea lotus L. petal extracts in rats red blood cells. Nymphaea lotus L. petal was extracted with distilled water, 50% ethanol and 95% ethanol. The extracts contained total phenol contents of 485.77 ± 15.70 mg/g, 660.43 ± 16.65 mg/g and 652.77 ± 18.66 mg/g respectively. Their total flavonoid contents were 17.40 ± 4.67 mg/g, 48.39 ± 6.21 mg/g and 42.10 ± 5.07 mg/g, respectively. DPPH scavenging activity was represented by IC50 values of 15.42±0.78 µg/ml, 8.35 ± 0.44 µg/ml and 8.96 ± 0.78 µg/ml, respectively. The ferric antioxidant activity of the distilled water extract was 1.31g. In 50% ethanol, it was 1.24 g and in 95% ethanol, it was 1.36g. This is similar to vitamin C at 1 g. The hemolysis protection value presented by the IC50 was 114.66 ± 5.73µg/ml, 45.37 ± 3.40 µg/ml and 69.45 ± 5.16µg/ml, respectively. These studies revealed the antioxidant activity and protective effects afforded cell membranes from free radicals by Nymphae lotus L. petal extracts. This was due to their rich composition of phenolic compounds.
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