Effect of Apis Dorsata honey and honey sugars analogue on hematological and some biochemical parameters in albino rats model
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Published:
Apr 19, 2018
Keywords:
Tualang honey honey sugars analogue haematological parameters
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Abstract
Honey has been recognized for its wound-healing, anti-microbial, anti-oxidant, anti-diabetic and anticancer effects. This study was designed to examine the effect of daily oral administration of Malaysian Tualang honey (TH) and honey sugars analogue (HSA) namely fructose, glucose, maltose and sucrose in albino Sprague-Dawley (SD) rats model. Thirty nulliparous female rats were divided into three groups: Group 0 (negative control); Groups 1 and 2 received 1.0 g/kg body weight/day of TH and HSA respectively. After 120 days treatment, the rats were subjected to necropsy and blood samples were collected for analyses. The body weight, fasting blood glucose, haematological parameters and serum level expression of proteins such as Apaf-1, IFN-????, TNF-ɑ and E2 were determined. Results show that an increased body weight was observed as the treatment progressed over days (p˃0.05). TH and HSA showed an increasing effect on the level of haematological parameters such as red blood cells (RBC), haemoglobin (Hb), packed cell volume (PCV) (p˃0.05), lymphocytes, TWBC, RDW, eosinophils, monocytes and platelets (p˂0.05) compared to the non-treated negative control. HSA showed a slightly comparable effect to the negative control for fasting blood glucose level, while TH presented a slightly hypoglycemic effect (p˃0.05). The treatments also showed an increasing effect on serum level of Apaf-1, IFN-????, TNF-ɑ and a reduced level for E2. In conclusion, daily supplementation of TH and HSA may modulate haematological and serological parameters.
Article Details
How to Cite
Ahmed, S., Sulaiman, S. A., & Othman, N. H. (2018). Effect of Apis Dorsata honey and honey sugars analogue on hematological and some biochemical parameters in albino rats model. Asia-Pacific Journal of Science and Technology, 23(2), APST–23. https://doi.org/10.14456/kkurj.2018.4
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Research Articles
References
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[2] Al-rahbi, B., Zakaria, R., Othman, Z., Hassan, A., Ahmad, A. H., 2014. Enhancement of BDNF Concentration and Restoration of the Hypothalamic-Pituitary-Adrenal Axis Accompany Reduced Depressive-Like Behaviour in Stressed Ovariectomised Rats Treated with Either Tualang Honey or Estrogen. The Scientific World Journal 2014, 8.
[3] Bogdanov, S., Jurendic, T., Sieber, R., Gallmann, P., 2008. Honey for nutrition and health: a review. Journal of the American College of Nutrition 27, 677-689.
[4] Miraglio, A. M., 2008. honey-health and therapeutic qualities. The National Honey Board.
[5] Ahmed, S., Othman, N. H., 2013b. Review of the medicinal effects of tualang honey and a comparison with manuka honey. Malays J Med Sci 20, 6-13.
[6] Bashkaran, K., Zunaina, E., Bakiah, S., Sulaiman, S. A., Sirajudeen, K., Naik, V., 2011. Anti-inflammatory and antioxidant effects of Tualang honey in alkali injury on the eyes of rabbits: experimental animal study. BMC Complement Altern Med 11, 90.
[7] Sukur, S. M., Halim, A. S., Singh, K. K., 2011. Evaluations of bacterial contaminated full thickness burn wound healing in Sprague Dawley rats Treated with Tualang honey. Indian J Plast Surg 44, 112-117.
[8] Erejuwa, O. O., Sulaiman, S. A., Wahab, M. S. A., Sirajudeen, K. N. S., Salleh, M. S. M., Gurtu, S., 2011b. Differential Responses to Blood Pressure and Oxidative Stress in Streptozotocin-Induced Diabetic Wistar-Kyoto Rats and Spontaneously Hypertensive Rats: Effects of Antioxidant (Honey) Treatment. International Journal of Molecular Sciences 12, 1888-1907.
[9] Fauzi, A. N., Norazmi, M. N., Yaacob, N. S., 2011. Tualang honey induces apoptosis and disrupts the mitochondrial membrane potential of human breast and cervical cancer cell lines. Food Chem Toxicol 49, 871-878.
[10] Erejuwa, O. O., Sulaiman, S. A., Wahab, M. S., Sirajudeen, K. N., Salleh, M. S., Gurtu, S., 2011a. Glibenclamide or metformin combined with honey improves glycemic control in streptozotocin-induced diabetic rats. Int J Biol Sci 7, 244-252.
[11] Shin, H.-S., Ustunol, Z., 2005. Carbohydrate composition of honey from different floral sources and their influence on growth of selected intestinal bacteria: An in vitro comparison. Food Research International, 38 721-728.
[12] Wang, X. H., Andrae, L., Engeseth, N. J., 2002b. Antimutagenic effect of various honeys and sugars against Trp-p-1. J Agric Food Chem 50, 6923-6928.
[13] Speicher, T., Foehrenbacher, A., Pochic, I., Weiland, T., Wendel, A., 2010. Malignant but not naïve hepatocytes of human and rodent origin are killed by TNF after metabolic depletion of ATP by fructose. Journal of hepatology 53, 896-902.
[14] Park, A., 2009. All sugars aren’t the same: Glucose is better, study says. Time Magazine April, 21.
[15] Mandal, M. D., Mandal, S., 2011. Honey: its medicinal property and antibacterial activity. Asian Pacific Journal of Tropical Biomedicine 1, 154-160.
[16] Ames, B. N., Mccann, J., Yamasaki, E., 1975. Methods for detecting carcinogens and mutagens with the salmonella/mammalian-microsome mutagenicity test. Mutation Research/Environmental Mutagenesis and Related Subjects 31, 347-363.
[17] Maron, D. M., Ames, B. N. 1983. Revised methods for the Salmonella mutagenicity test. Mutat Res 113, 173-215.
[18] Skog, K., 1993. Cooking procedures and food mutagens: a literature review. Food Chem Toxicol 31, 655-675.
[19] Hueper, W. C., 1965. Are Sugars Carcinogens? An Experimental Study. Cancer Res 25, 440-443.
[20] Ferraro, E., Pesaresi, M. G., DE Zio, D., Cencioni, M. T., Gortat, A., Cozzolino, M., Berghella, L., Salvatore, A. M., Oettinghaus, B., Scorrano, L., pérez-payà, E., Cecconi, F., 2011. Apaf1 plays a prosurvival role by regulating centrosome morphology and function. Journal of Cell Science 124, 3450.
[21] Mcilwain, D. R., Berger, T., Mak, T. W., 2013. Caspase functions in cell death and disease. Cold Spring Harbor perspectives in biology 5, a008656.
[22] Cavalcanti, Y. V. N., Brelaz, M. C. A., Neves, J. K. D. A. L., Ferraz, J., Candido, Pereira, V., R, R., Alves, G., 2012. Role of TNF-Alpha, IFN-Gamma, and IL-10 in the Development of Pulmonary Tuberculosis. Pulmonary Medicine 2012, 10.
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[24] Medic, V., 2013. Modulation of Interleukin-17 By Tumor Necrosis Factor Alpha During the Immune Response to Borrelia Burgdorferi.
[25] Soucy, G., Boivin, G., Labrie, F., Rivest, S., 2005. Estradiol Is Required for a Proper Immune Response to Bacterial and Viral Pathogens in the Female Brain. The Journal of Immunology 174, 6391.
[26] Chao, T. C., Van alten, P. J., Greager, J. A., Walter, R. J., 1995. Steroid sex hormones regulate the release of tumor necrosis factor by macrophages. Cell Immunol 160, 43-49.
[27] Aliyu, M., Odunola, O. A., Owumi, S. E., Gbadegesin, M. A., Choudhary, M. I., Faroq, A., Rasheed, H., Erukainure, O. L., Ahmed, S., 2012c. Daily consumption of honey: effects on male wister albino rats. International Journal of Food and Safety 1, 66-74.
[28] Allsop, K. A., Miller, J. B., 1996. Honey revisited: a reappraisal of honey in pre-industrial diets. Br J Nutr 75513-75520.
[29] Chen, L., Mehta, A., Berenbaum, M., Zangerl, A. R., Engeseth, N. J., 2000. Honeys from Different Floral Sources as Inhibitors of Enzymatic Browning in Fruit and Vegetable Homogenates. Journal of Agricultural and Food Chemistry 48, 4997-5000.
[30] Sherlock, O., Dolan, A., Athman, R., Power, A., Gethin, G., Cowman, S., Humphreys, H., 2010. Comparison of the antimicrobial activity of Ulmo honey from Chile and Manuka honey against methicillin-resistant Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. BMC Complement Altern Med 10, 47.
[31] Wang, X. H., Andrae, L., Engeseth, N. J., 2002b. Antimutagenic effect of various honeys and sugars against Trp-p-1. J Agric Food Chem 50, 6923-6928.
[32] Cooper, R. A., Molan, P. C., Krishnamoorthy, L., Harding, K. G., 2001. Manuka honey used to heal a recalcitrant surgical wound. Eur J Clin Microbiol Infect Dis 20, 758-759.
[33] Erejuwa, O. O., Sulaiman, S. A., Wahab, M. S., Sirajudeen, K. N., Salleh, M. S., Gurtu, S., 2010. Antioxidant protection of Malaysian tualang honey in pancreas of normal and streptozotocin-induced diabetic rats. Ann Endocrinol (Paris) 71, 291-296.
[34] Erejuwa, O. O., Sulaiman, S. A., Wahab, M. S., 2012b. Honey--a novel antidiabetic agent. Int J Biol Sci 8, 913-934.
[35] Tomasin, R., Gomes-marcondes, M. C., 2011. Oral administration of Aloe vera and honey reduces Walker tumour growth by decreasing cell proliferation and increasing apoptosis in tumour tissue. Phytother Res 25, 619-623.
[36] Ahmed, S., Othman, N. H., 2013a. Honey as a Potential Natural Anticancer Agent: A Review of Its Mechanisms. Evid Based Complement Alternat Med 2013, 829070.
[37] Aliyu, M., Odunola, O. A., Owumi, S. E., Gbadegesin, M. A., Choudhary, M. I., Farooq, A. D., Rasheed, H., erukainure, O. L., Ahmed, S., 2012b. Daily consumption of honey: effects on male wister albino rats. International Journal of Food Nutrition and Safety 1, 66-74.
[38] Caan, B. J., Kwan, M. L., Hartzell, G., Castillo, A., Slattery, M. L., Sternfeld, B., Weltzien, E., 2008. Prediagnosis
body mass index, post-diagnosis weight change, and prognosis among women with early stage breast cancer. Cancer Causes Control 19, 1319-13128.
[39] Ron, F., 2007. Honey - more than a sweetener, naturally. World Class Emprise, LLC, in the United States [WWW Document] URL https://www.worldclassemprise.com/custom.aspx?id=22 2007. 1.
[40] AkinbamI, A., Popoola, A., Adediran, A., DOSUNMU, A., Oshinaike, O., Adebola, P., Ajibola, S., 2013. Full blood count pattern of pre-chemotherapy breast cancer patients in Lagos, Nigeria. Caspian J Intern Med 4, 574-579.
[41] Sheikh, N., Masood, M., Naz, N., 2011. Hematological and serological changes in the pre- and posttreatment breast cancer patients. HealthMED 5.
[42] Aliyu, M., Odunola, O. A., Owumi, S. E., Gbadegesin, M. A., Choudhary, M. I., Farooq, A. D., Rasheed, H., Erukainure, O. L., Ahmed, S., 2012b. Daily consumption of honey: effects on male wister albino rats. International Journal of Food Nutrition and Safety 1, 66-74.
[43] Zou, H., Henzel, W. J., Liu, X., Lutschg, A., Wang, X., 1997. Apaf-1, a Human Protein Homologous to C. elegans CED-4, Participates in Cytochrome c–Dependent Activation of Caspase-3. Cell 90, 405-413.
[44] Campioni, M., Santini, D., Tonini, G., Murace, R., Dragonetti, E., Spugnini, E. P., Baldi, A., 2005. Role of Apaf-1, a key regulator of apoptosis, in melanoma progression and chemoresistance. Experimental Dermatology 14, 811-818.
[45] Igney, F. H., Krammer, P. H., 2002. Death and anti-death: tumour resistance to apoptosis. Nat Rev Cancer 2, 277-288.
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